A Study of Colonization with ESBL and AMPC E Coli in Gut of Patients of Tertiary Care Hospital, Ahmedabad
Keywords:
ESBL, E.coli, AmpCAbstract
Background: Higher prevalence of ESBL (Extended spectrum betalacamase)-producing E. coli in fecal carriage has been reported in the nosocomial setting than in the community due to high levels of antibiotic consumption. This study is to know the prevalence of ESBL producing E.coli in stool samples of Hospitalized patient with non GIT complains.
Material & Methods: A total 300 Stool samples of the patients with non GIT complains admitted in General Hospital, Sola, Ahmadabad were collected for study between December 2012 to March 2013. All samples were tested for routine stool microscopy as well as culture and sensitivity according to NCCLS guideline.
Result: Of 100 samples showing E.coli out of 300 samples, 43% show ESBLs producing E.coli and Amp C producing E.coli show in 26% cases. While 31% was normal E.coli without ESBLs B-lactamase and AmpC B lactamase. Commonest age group is 0-15 years.
Conclusion: Our normal gut flora is highly replaced by the ESBLs/AmpC producing E.coli, though they are non pathogenic organism. A threatening epidemiological problem is the dissemination of ESBL-producing organisms to healthy people in the community, which might depend on the frequency of ESBL fecal carriage as well as on the presence of ESBL producing organisms in the food chain.
References
Huge Edgardo Villar, Marisa Noemi Baserni, Monica Bearnos Aiers, Argentina Faecal carriage of ESBL pro-ducing Enterobactericae and Carbapenam resistant Gram negative bacilli in community setting. Infect Dev Ctries 2013;7(8):630-634.
A.A.Kader and K.A.Kamath, Faecal carriage of extend-ed spectrum B lactamase producing bacteria in the community. Eastern Mediterrannean Health Journal, Vol.15, No.6, 2009.1365-1369.
Bradford PA. Extended-spectrum β-lactamases in the 21st century: characterization, epidemiology, and detec-tion of this important resistance threat. Clinical microbi-ology reviews, 2001, 14:933–51.
VAnil Kumar and Rachana Babu ,Faecal carriage of extended spectrum B lactamase producing Entero-cateriaceae,J Med Microb Diagn .2013 .2(3).1-2.
Moland SE, Black JA. Ourada J, Reisbig MD, Hanson ND, Thomson KS. Occurrence of newer b-lactamases in Klebsiella pneumoniae isolates from 24 US hospitals. An-timicrob Agents Chemother 2002; 46:3837-3842.
Kassis-Chikhani N. Vimont S, Asselat K, Trivalle C, Minassian B, et al. (2004) CTX-M β-lactamase produc-ing Escherichia coli in long-term care facilities, France. Emerg Infect Dis 10: 1697-1698.
Bradford PA. Urban C, Jaiswal A, Mariano N, Ras-musssen BA, et al. (1995) SHV-7, a novel cefotaxime-hydrolyzing β-lactamase, identified in Escherichia coli isolates from hospitalized nursing home patients. Anti-microb Agents Chemother 39: 899-905.
Canton, R. and T. M. Coque. 2006. The CTX-M β-lactamase pandemic. Curr. Opin. Microbiol. 9466-475. [PubMed]
Paterson, D. L. and R. A. Bonomo. 2005. Extended-spectrum β-lactamases: a clinical update. Clin. Microbi-ol. Rev. 18657-686. [PMC free article] [PubMed]
Curtis J. Donskey ,Antibiotic Regimens and Intestinal Colonization with Antibiotic-Resistant Gram –Negative Bacilli.Clinical Infectious Diseases 2006;43:S62-9.
Cheesbrough M, District Laboratory Practice in Tropical Countries Part2; 2006; Pg.124- 130
Collee Et Al. Mackie & McCartney Practical Medical Microbiology, 14th edition Pg. 95-112; 113-130; 131-150; 151-178; 361-384.
Koneman EW Et Al. Color Atlas and Textbook of Diag-nostic Microbiology, 6th edition.Pg: 946-1014; 213-293; 97-105.
NCCLS guideline, 2012, page no: 15-20
Qiang Sun, Maria Tarnberg, Lingbo etal. Varying High Levels of Fecal Carriage of Extended Spectrum Beta –Lactamase Producing Enterobacteriaceae in Rural Vil-lages in Shandong,China: Implications for Global Health .PloS ONE9(11):2014.
Arazazu Valvade, High rate of Intestinal Colonization with Extended-spectrum-B lactamase-Producing Or-ganisms in Household Contacts of Infected Community Patients,Auguest 2008; 46(8) 2796-2799.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
The authors retain the copyright of their article, with first publication rights granted to Medsci Publications.
