Comparison Of Double Disc Diffusion Method and Vitek 2 Compact System to Screen the ESBL Producers in Intensive Care Unit in Hospital

Authors

  • Kinal Shah U.N Mehta Institute of Cardiology & Research centre, Ahmedabad
  • Gaurishanker Shrimali GMERS Medical College, Gandhinagar, Gujarat
  • Summaiya Mulla Government Medical College, Surat

Keywords:

ESBL, Double disk diffusion, Vitek 2 compact, E. coli, Klebsiella pneumoniae

Abstract

Background: It is essential to develop screening methods for the detection of Extended Spectrum of B- Lactamase (ESBL) producing strains in the laboratories, so that the appropriate medication can be started.  In this study we compared the ESBL strain detection with the help of double disk diffusion method and Vitek 2 compact (Biomerieux India Pvt. Ltd.).

Material and Methods: 67 clinical isolates of E. coli (33) and Klebsiella spp. (34) were isolated from the intensive care unit of the hospital from India and screened for ESBL production by double disc diffusion method and Vitek 2 compact system (fully automated susceptibility testing and identification system).

Results:  Total 67 isolates of E. coli and Klebsiella spp. were screened in this study, out of that 16 isolates E. coli (9) and Klebsiella (7) were ESBL non-producer according to double disk diffusion method, and then these 16 isolates were retested by Vitek 2 compact system.  Out of these16, 14 were ESBL non-producer E. coli (7) and Klebsiella (7) and 2 were ESBL producer by Vitek 2 compact.

Conclusion: From the above results we can conclude that Vitek 2 compact is an automated system and gives more accurate results than double disk diffusion method.

References

Du Bois, S. K., Marriott, M. S. & Amyes, S. G. B. (1995). TEM- and SHV-derived extended-spectrum ß-lactamases: relationship between selection, structure and function. J Antimicrob Chemother 35, 7–22.

Heritage, J., M'Zali, F. H., Gascoyne-Biniz, D. & Hawkey, P. M. (1999). Evolution and spread of SHV extended-spectrum ß-lactamases in Gram-negative bacteria. J An-timicrob Chemother 44, 309–318.

Sanders, C. C., Barry, A. L., Washington, J. A., Shubert, C., Moland, E. S., Traczewski, M. M., Knapp, C. & Mulder, R. (1996). Detection of extended-spectrum ß-lactamase-producing members of the family Enterobacte-riaceae with the Vitek ESBL test. J Clin Microbiol 34, 2997–3001.

Bush, K., Jacoby, G. A. & Medeiros, A. A. (1995). A func-tional classification scheme for ß-lactamases and its cor-relation with molecular structure. Antimicrob Agents Chemother 39, 1211–1233.

Bradford, P. A., Urban, C., Jaiswal, A., Mariano, N., Rasmussen, B. A., Projan, S. J., Rahal, J. J. & Bush, K. (1995). SHV-7, a novel cefotaxime-hydrolyzing ß-lactamase identified in E. coli isolated from hospitalized nursing home patients. Antimicrob Agents Chemother 39, 899–905.

Hawkey, P. M. & Munday, C. J. (2004). Multiple re-sistance in Gram-negative bacteria. Rev Med Microbiol 15, 51–61.

National Committee for Clinical Laboratory Standards (1999). Performance Standards for Antimicrobial Suscep-tibility; Ninth Informational Supplement M100-S9. Villa-nova, PA, USA: National Committee for Clinical Labora-tory Standards.

A.A. Dashti, P. West, R. Paton, S.G.B. Amyes (2006). Characterisation of extended spectrum ß -lactamase (ESBL) producing Kuwait and UK strains identified by the Vitek system, and subsequent comparison of the Vi-tek system with other commercial ESBL-testing system using these strains.

Thomson, K. S. & Sanders, C. C. (1992). Detection of ex-tended-spectrum ß-lactamases in members of the family Enterobacteriaceae – comparison of the double-disk and 3-dimensional tests. Antimicrob Agents Chemother 36, 1877–1882.

Gibb, A. P. & Crichton, M. (2000). Cefpodoxime screen-ing of Escherichia coli and Klebsiella spp. by Vitek for de-tection of organisms producing extended-spectrum ß-lactamases. Diagn Microbiol Infect Dis 38, 255–257.

Downloads

Published

2016-09-30

How to Cite

1.
Shah K, Shrimali G, Mulla S. Comparison Of Double Disc Diffusion Method and Vitek 2 Compact System to Screen the ESBL Producers in Intensive Care Unit in Hospital. Natl J Community Med [Internet]. 2016 Sep. 30 [cited 2024 Mar. 28];7(09):789-91. Available from: https://njcmindia.com/index.php/file/article/view/1058

Issue

Section

Original Research Articles