Association Of the Methylene Tetrahydrofolate Reductase (MTHFR) Gene Polymorphism with Susceptibility to Recurrence of Cardiovascular Outcomes Among Ischemic Stroke- A Randomized Controlled Trial

Neetu Kataria; Vasantha C Kalyani; Anissa Atif Mirza; Vivekanandhan S; Mritunjai Kumar; Yogesh Bharupi; Shashi Ranjan; Nitesh Kumar; Niraj Kumar

doi:10.55489/njcm.131020222420

Authors

Neetu Kataria All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India
Vasantha C Kalyani All India Institute of Medical Sciences (AIIMS), Deoghar, Jharkhand, India
Anissa Atif Mirza All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India https://orcid.org/0000-0002-7531-3445
Vivekanandhan S All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India
Mritunjai Kumar All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India https://orcid.org/0000-0002-4113-9186
Yogesh Bharupi All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India https://orcid.org/0000-0003-2433-1624
Shashi Ranjan All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India https://orcid.org/0000-0003-3206-7755
Nitesh Kumar All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India https://orcid.org/0000-0003-4065-6058
Niraj Kumar All India Institute of Medical Sciences (AIIMS), Rishikesh, Uttarakhand, India https://orcid.org/0000-0002-5863-2532

DOI:

https://doi.org/10.55489/njcm.131020222420

Keywords:

Gene polymorphism, Ischemic stroke, homocysteine, recurrence, cardiovascular disorders

Abstract

Background: Hyperhomocystenemia and genetic variants are factors for causing young age stroke globally. This study aims to identify homocysteine related-MTHFR gene polymorphism that associated with recur-rent cardiovascular outcomes.

Methodology: A randomized controlled trial conducted upon 90 hyperhomocysteinemic ischemic stroke patients were taken from the neurology wards of a tertiary care hospital were randomly selected into vita-min B therapy group and control groups (n=45 in each group). Baseline subject details were collected ve-nous blood sample for MTHFR genetic testing via PCR-RFLP technique along with blood homocysteine lev-els, vitamin B12, folic acid levels.

Results: The results showed that the frequency of CT genotype polymorphism was 15.5% vs 13.3% for the MTHFR C677T gene without any significant difference between vitamin group and control group respective-ly (p-value >0.05). The reduction in mean homocysteine up to -6.77±4.50 versus -2.08±0.71 µmol/L in the vitamin group as compared to control group respectively, p value 0.001.

Conclusion: Considerable amount of MTHFR gene polymorphism found among hyperhomocysteinemic is-chemic stroke of sub-Himalayan region. Nutritional deficiencies including vitamin B 12 & folic acid, and some hidden reasons found, which could lead to the primary cause of hyperhomocysteinemia. Vitamin B therapy is an effective for reducing homocysteine.

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