Study Of Hemoglobinopathies in Patients of Anemia Using High Performance Liquid Chromatography (HPLC) In Western India

Authors

  • Jignasa N Bhalodia GMERS Medical College, Sola, Ahmedabad
  • Haren V Oza GMERS Medical College, Sola, Ahmedabad
  • Palak J Modi GMERS Medical College, Sola, Ahmedabad
  • Ami M Shah GMERS Medical College, Sola, Ahmedabad
  • Kinara A Patel GMERS Medical College, Sola, Ahmedabad
  • Himani B Patel GMERS Medical College, Sola, Ahmedabad

Keywords:

Anaemia, Hemoglobinopathies, HPLC

Abstract

Introduction: In India, major cause of anaemia is nutritional deficiencies which can be treated by medications. Hemoglobinopathies are the most common inherited red cell disorders worldwide. Most clinically significant hemoglobinopathies are inherited defects of the beta (β) globin chain of adult haemoglobin. Identification of these disorders is immensely important epidemiologically and for improved management protocols. Our aim is to detect the haemoglobin disorders in patients with anaemia and to assess the suitability of using high performance liquid chromatography (HPLC) routinely for screening patients with anaemia.

Methods: A total of 500 cases of patient’s with anaemia having haemoglobin up to 11 gm% were studied for routine haematological investigations and by HPLC for diagnosing any abnormal haemoglobin disorder by BIO RAD ‘VARIANT’ analyser.

Results: Out of 500 cases of anaemia studied, 43 cases showed abnormal haemoglobin fractions on HPLC. Of these, 26 cases of the beta Thalassemia trait was the predominant abnormality (5.2%). There were 6 cases of sickle cell trait patients (1.2%), 4cases of high Hb F patients (0.8%) and 2 cases of Hb D Punjab heterozygous patients (0.4%). Other hemoglobinopathies were also identified in smaller proportions.

Conclusion: HPLC is very simple, accurate and superior technique in early detection of various haemoglobin disorders, which helps in early management of patients.

References

Patel A P, Naik M R, Shah N M, Sharma N P, Parmar P H. Prevalence of common hemoglobinopathies in Gujarat: An analysis of a large population screening programme. National journal of community medi-cine. Jan- March 2012; Vol 3 (1): 112-116.

Parikh U R, Goswami H M, Mehta R C, Patel P S, Gosai R N. incidence of hemoglobinopathies in women attending antenatal clinics in their first trimester. NHL journal of medical science. Jan 2014; vol 3 (1): 63-67.

Ahmed A, Naser A, Talib Hassan A, MoayedNaji M. Detection of hemoglobinopathies in hypochromic, mi-crocytic and sickeled cell blood films by hemoglobin electrophoresis. Thi-Qar medical journal (TQMJ): 2011; vol 5 (1): 139-148.

Aziz B, Abolghasem P, Reza E. Discrimination of beta-thalssemia minor and iron deficiency anemia by screening test for blood cell indices. Turk J Med Sci. 2012; 42 (2): 275-280.

Baruah M K, Saikia M, Baruah A. pattern of hemo-globinopathies and thalassemias in upper Assam re-gion of North Eastern India: High performance liquid chromatography studies in 9000 patients. Indian journal pf pathology and microbiology. April-June 2014; 57(2): 236-243.

Patel U, Shrivastav A, Joshi J R, Agnihotri A S, Kaur A, Thakkar B. Detection of hemoglobinopathies and thalassemias in population of Gujarat state using HPLC: Analysis of 2022 cases. July- Dec 2012; Vol 4: Issue 2: 80-84.

Philip J, Sarkar R V, Kushwaha N. Microcytic hypo-chromic anemia: Should high performance liquid chromatography be used routinely for screening ane-mic and antenatal patients. Indian journal of pathol-ogy and microbiology. April-June 2013; 56(2): 109-113.

Joutovsky A, Hadzi-Nesic J, Nardi M. HPLC reten-tion time as diagnostic tool for hemoglobin variants and hemoglobinopathies: A study of 60,000 samples in clinical diagnostic laboratory. Clinical chemistry. 2004; 50 (10): 1736-1747.

Tyagi S, Saxena R, Choudhry V P. HPLC- how neces-sary is it for hemoglobinopathy diagnosis in India? Indian journal of pathology and microbiology. 2003; 46: 390-3.

Greer J P, Foerster J, Rodgers G M, Paraskevas F, Glader B, Arber D A et al. Wintrobes’ Clinical Hema-tology, Twelfth edition. China: Lippincott Williams & Wilkins; 2004. Pg. 1089-1090.

Patne S C U, Shukla J. Hemoglobin E disorders in Eastern Uttar Pradesh. Indian journal of pathology and microbiology. Jan- March 2009; 52(1): 110-112.

Fakher R. Microcytic hypochromic anemia patients with thalassemia: Genotypic approach. Indian J Med Sci. March 2009; 63 (3): 101-108.

Sachdev R, Dam A R, Tyagi G. Detection of Hb vari-ants and hemoglobinopathies in Indian population using HPLC: Report of 2600 cases. Indian journal of pathology and microbiology. Jan-March 2010; 53 (1): 57-62.

Patel A G, Shah A P, Sorathiya S M, Gupte S C. he-moglobinopathies in South Gujarat population and incidence of anemia in them. Indian Journal of Hum Genet. Sep-Dec 2012; 18(3): 294-298.

Madan N, Beachler L, Konstantinopoulos P, Worley S, Sun Z, Latson L A. Red cell indices and discriminant functions in the detection of beta-thalassaemia trait in a population with high prevalence of iron deficiency anaemia. PediatrCardiol. 2010; 31: 1203-8.

Dash S, Kumar S, Dash R J. Hematological malignan-cy in hemoglobin D disease. Am J Hematol 1988; 27: 305.

Downloads

Published

2015-03-31

How to Cite

1.
Bhalodia JN, Oza HV, Modi PJ, Shah AM, Patel KA, Patel HB. Study Of Hemoglobinopathies in Patients of Anemia Using High Performance Liquid Chromatography (HPLC) In Western India. Natl J Community Med [Internet]. 2015 Mar. 31 [cited 2024 Nov. 21];6(01):35-40. Available from: https://njcmindia.com/index.php/file/article/view/1125

Issue

Vol. 6 No. 01 (2015)

Section

Original Research Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

The authors retain the copyright of their article, with first publication rights granted to Medsci Publications.