Study Of Protein Carbonyl Group, Nitric Oxide and MDA (Index of Lipid Peroxidation) As Biomarkers of Oxidative Stress in Type 2 Diabetes Mellitus
Keywords:
Diabetes mellitus, oxidative stress, protein carbonyl (CO) group, nitric oxide (NO), malondialdehyde (MDA)Abstract
Background: Diabetes mellitus (DM) is metabolic disorders characterized by hyperglycemia and abnormalities in lipid and protein metabolism. The free radicals and oxidative stress may act as a common pathway to diabetes itself, as well as to its later complications.
Objectives: The present study was planned to study the biomarkers of oxidative stress, such as protein carbonyl (CO) group, nitric oxide (NO.) in the form of total nitrite (NOx) and malondialdehyde (MDA) as an index of lipid peroxidation in type 2 DM patients and healthy individuals.
Methodology: We studied 60 cases of type 2 DM and 30 healthy individuals as control. Serum protein carbonyl estimated by dinitrophenyl hydrazine (DNPH) method, nitrate (NO3-) and nitrite (NO2- ) concentrations in terms of total nitrites (NOx) by Griess reaction and MDA by thiobarbituric acid reagent test.
Results: We found highly significant increase in the level (Mean ± SD) of protein carbonyl (3.28 ± 0.75 nmol/mg), NOx (total nitrites) (176 ± 68.12 mmol/L) and MDA (8.3 ± 1.19 nmol/ml) in type 2 DM patients compared to healthy subjects (1.51 ± 0.22 nmol/mg), (46.6 ± 17.5 mmol/L) and (3.12 ± 0.77 nmol/ml) respectively (P < 0.0001).
Conclusion: Biomarkers of oxidative stress such as protein carbonyl, NOx (total nitrites) and MDA are significantly increased in type 2 DM compared to healthy subjects. There is increased protein oxidation, lipid peroxidation, and increased conversion of NO. to its end products like nitrite and nitrates in type 2 DM. This suggests that there is increased production of reactive oxygen species (ROS) and oxidative stress in type 2 DM patients compared to healthy subjects.
References
Abou-Seif MA, Youssef AA. Evaluation of some biochemical changes in diabetic patients. Clin Chim Acta. 2004; 346 (2): 161-70.
Osawa T. Kato Y. Protective role of antioxidative food factors in oxidative stress caused by hyperglycemia. Ann N Y Acad Sci. 2005 Jun;1043:440-51.
Marshll WJ, Bangert SK. Clinical Biochemistry, Meta-bolic and Clinical aspects, 2nd edn. Philadelphia PA: Churchill Livingstone Elsevier; 2008. pp. 942-951.
Abuja PM, Albertini R. Methods for monitoring oxidative stress, lipid peroxidation and oxidation re-sistance of lipoproteins. Clin Chim Acta. 2001 Apr;306(1-2):1-17.
Odetti P, Garibaldi S, Noberasco G, Aragno I, Valentini S, Traverso N, et.al. Levels of carbonyl groups in plas-ma proteins of type 2 diabetes mellitus subjects. Acta Diabetologica.1999 Dec; 36(4):179-83.
Bhagavan NV. Medical Biochemistry, 4th edn. Burling-ton, Masasachusetts 01803: Harcourt / Academic Press; 2002. pp. 345-47.
Choi JW, Im MH, Pai SH. Nitric oxide production increases during normal pregnancy and decreases in preeclampsia. Ann Clin Lab Sci.2002; 32(3):257-63.
Moshage H. Nitric oxide determination: much ado about NO-thing? Clin Chem. 1997; 43 (4):553 -56.
Devasagayam TP, Boloor KK, Ramasarma T. Methods for estimating lipid peroxidation: an analysis of merit and demerits. Indian J Biochem Biophys. 2003 Oct; 40(5):300-8.
Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, et.al. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol.1990; 186: 464-78.
Dusse LM, Silva RM, Vieira LM, das Graças Carvalho M. Does plasma nitrite determination by Griess reac-tion reflect nitric oxide synthesis. Clin Chim Ac-ta.2005Dec; 362(1-2):195-197.
Wilbur KM, Bernheim F, Shapiro OW. The thiobarbitu-ric acid reagent as a test for oxidation of unsaturated fatty acids by various reagents. Arch Biochem.1949 Dec; 24(2): 305-313.
Maritim AC. Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 2003;17(1):24-38.
Bonnfont-Rousselot D, Bastard JP, Jaudon MC, Delatre J. Consequences of the diabetic status on the oxidant / antioxidant balance. Diabetes Metab. 2002 May; 26(3):163-176.
Caimi G, Carollo C, Lo Presti R. Diabetes mellitus: oxidative stress and wine. Curr
Med Res Opin. 2003; 19(7): 581-586.
Fauci AS, Braunwald E, Isselbacher KJ, Wilson JD, Martin JB, Kasper DL et al. Harrison’s principles of internal medicine, 14th edn (International edn), New York: McGraw-Hill; 1998. pp.442-444.
Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarker of oxidative stress. Clin Chim Acta. 2003 Mar;329 (1-2):23-28.
Cakatay U. Protein oxidation parameters in type 2 diabetic patients with good and poor glycemic control. Diabetes Metab. 2005 Dec;31(6):551-7.
Martin-Gallan P, Carrascosa A, Gussinye M, Dominguez C. Biomarkers of diabetes-associated oxidative stress and antioxidant status in young dia-betic patients with or without subclinical complica-tions. Free Radic Biol Med. 2003 Jun 15; 34(12):1563-74.
Telci A, Cakatay U, Salman S, Satman I, Sivas A. oxidative protein damage in early stage Type 1 diabet-ic patients. Diabetes Res Clin Pract. 2000 Dec; 50(3):213-23.
Moncanda S, Higgs A. The L-arginine: Nitric oxide pathway. N Eng J Med. 1993 Dec 30; 329(27):2002-12.
Honing ML, Morrison PJ, Banga JD, Stroes ES, Rabelink TJ. Nitric oxide availability in diabetes mellitus. Diabe-tes Metab Rev.1998 Sep; 14(3):241-9.
Bonnefont-Rousselot D. Glucose and reactive oxygen species. Curr Opin Clin Nutr Metab Care. 2002 Sep; 5(5):561-8.
Aggard E. Nitric oxide: mediator, murderer, and medicine. Lancet.1994 May14; 343(8907):1199-206.
Avogaro A, de Kreutzenberg SV. Mechanisms of endothelial dysfunction in obesity. Clin Chim Acta. 2005; 360 (1-2): 9-26.
Cosentino F, Hishikawa K, Katusic ZS, Luscher TF. High glucose increases nitric oxide synthase expression and superoxide anion generation in human aortic endo-thelial cells. Circulation. 1997; 96:25-28.
Moshage H, Kok B, Huizenga JR, Jansen PL. Nitrite and nitrate determination in plasma: a critical evalua-tion. Clin Chem. 1995 Jun; 41(6):892-96.
Garg MC, Choudhary DP, Bansal DD. Effect of vitamin E supplementation on diabetes induced oxida-tive stress in experimental diabetes in rat. Indian J Exp Biol. 2005 Feb; 43(2):177-80.
Atli T, Keven K, Avci A, Kutlay S, Turkcapar N, Varli M, et al. Oxidative stress and antioxidant status in elderly diabetes mellitus and glucose intolerance pa-tients. Arch Gerontol Geriatr. 2004 Nov-Dec; 39(3):269-75.
Likidlilid A, Patchanans N, Peerapatdit T, Sriratanasathavorn C. Lipid peroxidation and anti-oxidant enzyme activities in erythrocytes of type 2 dia-betic patients. J Med Assoc Thai. 2010 Jun; 93(6):682-93.
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