Detection of Blastocystis hominis and Assessment of Personal Hygiene Practice in Elementary School Children in Kampa District, Kampar Regency, Riau, Indonesia
DOI:
https://doi.org/10.55489/njcm.160420254933Keywords:
Blastocystis, children, feces, elementary schoolAbstract
Background: Blastocystis is an opportunistic intestinal protozoan and the pathogenicity of the disease is still unclear. It can cause malnutrition and disrupt growth and development in children. This study aims to detect Blastocystis and determine characteristics and personal hygiene practices.
Methodology: A cross-sectional study, with a sample of 104 students from 8 elementary schools in Kampa District. Detection of Blastocystis using stool culture with Jones Medium. Characteristics of subjects and risk factors using a questionnaire through guided interviews.
Results: The prevalence of Blastocystis was 45.2% and all of them had no symptoms. Most of them had unclean nails (70.2%), did not wash their hands with soap after defecating (57.7%). All students had good behavior in defecating. Generally, students drink from refillable water purchased at drinking water depots, 53.8% of students keep and have contact with pets. There was no significant relationship between personal hygiene and Blastocystis (p>0.05).
Conclusions: The prevalence of Blastocystis is quite high, although no significant relationship was found in this study, teachers and parents still need to pay attention to students' personal hygiene, such as keeping their nails clean and washing their hands with soap after defecating.
References
Abdulsalam AM, Ithoi I, Al-Mekhlafi HM, Khan AH, Ahmed A, Surin J, et al. Prevalence, predictors and clinical significance of Blastocystis sp. in Sebha, Libya. Parasites and Vectors. 2013;6:86. DOI: https://doi.org/10.1186/1756-3305-6-86 PMid:23566585 PMCid:PMC3626707
Asfaram S, Daryani A, Sarvi S, Pagheh AS, Hosseini SA, Saberi R, et al. Geospatial analysis and epidemiological aspects of human infections with Blastocystis hominis in Mazandaran Province, Northern Iran. Epidemiol Health. 2019;41:e2019009. DOI: https://doi.org/10.4178/epih.e2019009 PMid:30999737 PMCid:PMC6533553
Salehi M, Mardaneh J, Niazkar HR, Minooeianhaghighi M, Arshad E, Soleimani F, Mohammadzadeh A. Prevalence and Subtype Analysis of Blastocystis hominis Isolated from Patients in the Northeast of Iran. J Parasitol Res. 2021 Jan 13;2021:8821885. DOI: https://doi.org/10.1155/2021/8821885 PMid:33510903 PMCid:PMC7822675
Bodnia I, Pokhil S, Bodnia K, Pavliy V, Skoryk L. Distribution and frequency of Blastocystis sp. by methods of microscopy and cultivation in faeces of residents of Kahrkov Region. Georgian Med News. 2022;(328-329):85-89. PMID: 36318848.
Jalallou N, Iravani S, Rezaeian M, Alinaghizade A, Mirjalali H. Subtypes distribution and frequency of Blastocystis sp. isolated from diarrheic and non-diarrheic patients. Iran J Parasitol. 2017;12(1):63-8.
Kosik-Bogacka D, Lepczyńska M, Kot K, Szkup M, Łanocha-Arendarczyk N, Dzika E, et al. Prevalence, subtypes and risk factors of Blastocystis spp. infection among pre- and perimenopausal women. BMC Infect Dis. 2021;21:1125. DOI: https://doi.org/10.1186/s12879-021-06815-z PMid:34724915 PMCid:PMC8559403
Shams M, Shamsi L, Yousefi A, Sadrebazzaz A, Asghari A, Mohammadi-Ghalehbin B, et al. Current global status, subtype distribution and zoonotic significance of Blastocystis in dogs and cats: a systematic review and meta-analysis. Parasites and Vectors. 2022;15:225. DOI: https://doi.org/10.1186/s13071-022-05351-2 PMid:35733146 PMCid:PMC9215001
Sari IP, Benung MR, Wahdini S, Kurniawan A. Diagnosis and identification of Blastocystis subtypes in primary school children in Jakarta. J Trop Pediatr. 2018;64(3):208-14. DOI: https://doi.org/10.1093/tropej/fmx051 PMid:28977665
Dogruman-Al F, Simsek Z, Boorom K, Ekici E, Sahin M, Tuncer C, Kustimur S, Altinbas A. Comparison of methods for detection of Blastocystis infection in routinely submitted stool samples, and also in IBS/IBD Patients in Ankara, Turkey. PLoS One. 2010 Nov 18;5(11):e15484. DOI: https://doi.org/10.1371/journal.pone.0015484 PMid:21124983 PMCid:PMC2987810
Liu X, Ni F, Wang R, Li J, Ge Y, Yang X, et al. Occurrence and subtyping of Blastocystis in Coypus (Myocastor coypus) in China. Parasites and Vectors. 2022;15:14. DOI: https://doi.org/10.1186/s13071-021-05126-1 PMid:28049510 PMCid:PMC5209834
Directorate of Child Social Rehabilitation. The clean and healthy life behavior (PHBS). Jakarta: Ministry of Social Affairs of the Republic of Indonesia; 2020. 1-14 p. Available from: https://kemensos.go.id/uploads/topics/15863905705284.pdf
Sari IP, Wahdini S. Assessment of intestinal parasitic infection and personal hygiene practice among school children in a primary school in a village in Jakarta, Indonesia. Natl J Community Med. 2024;15(5):389-94. DOI: https://doi.org/10.55489/njcm.150520243796
Zman V, Khan KZ. A comparison of direct microscopy with culture for the diagnosis of Blastocystis hominis. Southeast Asian J Trop Med Public Health. 1994 Dec;25(4):792-3. PMID: 7667737.
Maryanti E, Lesmana SD, Firja W, Devlin M, Mislindawati M, Siagian FE. Detection of Blastocystis hominis by method of cultivation in the feces of orphanage children in Pekanbaru, Riau Province, Indonesia. J Biomed Transl Res. 2023;9(1):14-8. DOI: https://doi.org/10.14710/jbtr.v9i1.16470
Badparva E, Sadraee J, Kheirandish F, Frouzandeh M. Genetic diversity of human blastocystis isolates in khorramabad, central iran. Iran J Parasitol. 2014 Mar;9(1):44-9. PMID: 25642259; PMCID: PMC4289879.
Kumarasamy V, Rajamanikam A, Anbazhagan D, Atroosh WM, Azzani M, Subramaniyan V, et al. Systematic review and meta-analysis: epidemiology of human Blastocystis spp. infection in Malaysia. Trop Med Infect Dis. 2023;8(8):415. DOI: https://doi.org/10.3390/tropicalmed8080415 PMid:37624353 PMCid:PMC10457748
Alexeree SMI, Abou-Seri HM, EL-Din HES, Youssef D, Ramadan MA. Green synthesis of silver and iron oxide nanoparticles mediated photothermal effects on Blastocystis hominis. Lasers Med Sci. 2024;39(43). DOI: https://doi.org/10.1007/s10103-024-03984-6 PMid:38246979 PMCid:PMC10800310
Xu N, Jiang Z, Liu H, Jiang Y, Wang Z, Zhou D, et al. Prevalence and genetic characteristics of Blastocystis hominis and Cystoisospora belli in HIV/AIDS patients in Guangxi Zhuang Autonomous Region, China. Sci Rep. 2021;11:15904. DOI: https://doi.org/10.1038/s41598-021-94962-3 PMid:34354101 PMCid:PMC8342556
Zhang SX, Wang JC, Li ZW, Zheng JX, Zhou WT, Yang GB, et al. Impact factors of Blastocystis hominis infection in persons living with human immunodeficiency virus: a large-scale, multi-center observational study from China. Infect Dis Poverty. 2023;12:82. DOI: https://doi.org/10.1186/s40249-023-01137-5 PMid:37697423 PMCid:PMC10494452
Kök M, Çekin Y, Çekin AH, Uyar S, Harmandar F, Şahintürk Y. The role of Blastocystis hominis in the activation of ulcerative colitis. Turk J Gastroenterol. 2019 Jan;30(1):40-46. DOI: https://doi.org/10.5152/tjg.2018.18498. PMID: 30644363; PMCID: PMC6389295.
Fahim SM, Gazi MA, Hasan MM, Alam MA, Das S, Mahfuz M, et al. Infection with Blastocystis spp. and its association with enteric infections and environmental enteric dysfunction among slum-dwelling malnourished adults in Bangladesh. PLoS Negl Trop Dis. 2021;15(8):1-15. DOI: https://doi.org/10.1371/journal.pntd.0009684 PMid:34407080 PMCid:PMC8405003
Kumarasamy V, Atroosh WM, Anbazhagan D, Abdalla MMI, Azzani M. Association of Blastocystis hominis with colorectal cancer: A systematic review of in vitro and in vivo evidences. World J Gastrointest Oncol. 2022;14(3):734-45. DOI: https://doi.org/10.4251/wjgo.v14.i3.734 PMid:35321272 PMCid:PMC8919012
Nimri L, Batchoun R. Intestinal colonization of symptomatic and asymptomatic school children with Blastocystis hominis. J Clin Microbiol. 1994;32(11):2865-6. DOI: https://doi.org/10.1128/jcm.32.11.2865-2866.1994 PMid:7852590 PMCid:PMC264178
Dagci H, Kurt Ö, Demirel M, Mandiracioglu A, Aydemir S, Saz U, Bart A, VAN Gool T. Epidemiological and diagnostic features of blastocystis infection in symptomatic patients in izmir province, Turkey. Iran J Parasitol. 2014 Oct-Dec;9(4):519-29. PMID: 25759733; PMCID: PMC4345091.
Zaman Wahid B, Haque MA, Gazi MA, Fahim SM, Faruque ASG, Mahfuz M, et al. Site-specific incidence rate of Blastocystis hominis and its association with childhood malnutrition: findings from a multi-country birth cohort study. Am J Trop Med Hyg. 2023;108(5):887-94. DOI: https://doi.org/10.4269/ajtmh.22-0662 PMid:37037433 PMCid:PMC10160905
El Saftawy EA, Amin NM, Hamed DH, Elkazazz A, Adel S. The hidden impact of different Blastocystis genotypes on C-3 and IgE serum levels: a matter of debate in asthmatic Egyptian children. J Parasit Dis. 2019;43(3):443-51. DOI: https://doi.org/10.1007/s12639-019-01108-4 PMid:31406409 PMCid:PMC6667542
Sankur F, Ayturan S, Malatyali E, Ertabaklar H, Ertug S. The Distribution of Blastocystis Subtypes among School-aged Children in Mugla, Turkey. Iran J Parasitol. 2017 Oct-Dec;12(4):580-586. PMID: 29317883; PMCID: PMC5756308.
Udonsom R, Prasertbun R, Mahittikorn A, Mori H, Changbunjong T, Komalamisra C, et al. Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand. Infect Genet Evol. 2018;65:107-11. DOI: https://doi.org/10.1016/j.meegid.2018.07.007 PMid:30003970
Sanggari A, Komala T, Rauff-Adedotun AA, Awosolu OB, Attah OA, Farah Haziqah MT. Blastocystis in captivated and free-ranging wild animals worldwide: a review. Trop Biomed. 2022;39(3):338-72. DOI: https://doi.org/10.47665/tb.39.3.006 PMid:36214433
Onder Z, Yildirim A, Pekmezci D, Duzlu O, Pekmezci GZ, Ciloglu A, et al. Molecular identification and subtype distribution of Blastocystis sp. in farm and pet animals in Turkey. Acta Trop. 2021;220(March):105939. DOI: https://doi.org/10.1016/j.actatropica.2021.105939 PMid:33932364
Tadege B, Mekonnen Z, Dana D, Tiruneh A, Sharew B, Dereje E, Loha E, Ayana M, Levecke B. Assessment of the nail contamination with soil-transmitted helminths in schoolchildren in Jimma Town, Ethiopia. PLoS One. 2022 Jun 29;17(6):e0268792. DOI: https://doi.org/10.1371/journal.pone.0268792 PMid:35767573 PMCid:PMC9242460
Garn JV, Wilkers JL, Meehan AA, Pfadenhauer LM, Burns J, Imtiaz R, Freeman MC. Interventions to improve water, sanitation, and hygiene for preventing soil-transmitted helminth infection. Cochrane Database Syst Rev. 2022 Jun 21;6(6):CD012199. DOI: https://doi.org/10.1002/14651858.CD012199.pub2 PMid:35726112 PMCid:PMC9208960
Munn Z, Tufanaru C, Lockwood C, Stern C, McAneney H, Barker TH. Rinse-free hand wash for reducing absenteeism among preschool and school children. Cochrane Database Syst Rev. 2020 Apr 9;4(4):CD012566. DOI: https://doi.org/10.1002/14651858.CD012566.pub2 PMid:32270476 PMCid:PMC7141998
Fajar MM, Kurniawan MR, Wijayanti DR. Escherichia coli contamination in drinking water refills at Ciangsana Region using most probable number test. Borneo J Med Lab Technol. 2023;5(2):278-82. DOI: https://doi.org/10.33084/bjmlt.v5i2.4102
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Esy Maryanti, Yulia Wardany, Mislindawati, Luthfi Aulia Fadhlie, Atha Alwafa Balqis, Aida Rahmayeni

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
The authors retain the copyright of their article, with first publication rights granted to Medsci Publications.