Microbial Composition of Smokeless Tobacco Products from Karnataka
DOI:
https://doi.org/10.55489/njcm.13052022112Keywords:
smokeless tobacco, microbial, bacterialAbstract
Background: Though there is a lot of literature available about presence of carcinogenic chemicals in these products, there is lack of information regarding the microbial composition of these products.
Aim & Objective: The study was conducted with the objective of assessing microbial composition of smokeless tobacco products and factors associated with higher colony count.
Methodology: This cross sectional study included a total of 43 samples obtained from four districts of Karnataka namely Udupi, Bengaluru, Bidar and Belagavi. Microbial populations were assessed using quantitative aerobic culture and colony forming units per gram was reported for each sample. The data was entered into excel and analysed using Statistical Package for Social Sciences version 16.0. Results have been expressed as frequencies and percentages. Univarate analysis was done to study association between background characteristics and higher colony counts.
Results: All except three samples showed bacterial contamination. Two samples showed pathogenic bacterial growth, while the other 38 samples had non-pathogenic bacteria. There was no association between background characteristics and colony counts.
Conclusions: Microbial contamination of smokeless tobacco products is common. There is a need to further investigate various factors associated with microbial contamination so that necessary interventions can be implemented.
References
Tobacco market in India (2018-2023). Available at : https://www.researchandmarkets.com/reports/4757741/tobacco-market-in-india-2018-2023. Accessed on June 8th, 2021.
Prevalence of tobacco use in India. Available at https://www.mohfw.gov.in/sites/default/files/GATS-2%20FactSheet.pdf. Accessed on June 21st, 2021.
Aslesh OP, Paul S, Paul L, Jayasree AK. High prevalence of tobacco use and associated oral mucosal lesion among interstate male migrant workers in urban Kerala, India. Iran J Cancer Prev. 2015;8(6):e3876. doi: https://doi.org/10.17795/ijcp-3876 PMid:26855720 PMCid:PMC4736070
Thakur JS, Prinja S, Bhatnagar N, Rana S, Sinha DN, Singh PK. Socioeconomic inequality in the prevalence of smoking and smokeless tobacco use in India. Asian Pac J Cancer Prev. 2013;14(11):6965-9. doi: https://doi.org/10.7314/APJCP.2013.14.11.6965 PMid:24377634
Stepanov I, Jensen J, Hatsukami D, Hecht SS. New and traditional smokeless tobacco: comparison of toxicant and carcinogen levels. Nicotine Tob Res. 2008;10(12):1773-82. doi: https://doi.org/10.1080/14622200802443544 PMid:19023828 PMCid:PMC2892835
Wei X, Deng X, Cai D, Ji Z, Wang C, Yu J, et al. Decreased tobacco-specific nitrosamines by microbial treatment with Bacillus amyloliquefaciens DA9 during the air-curing process of burley tobacco. J Agric Food Chem. 2014;62(52):12701-6. doi: https://doi.org/10.1021/jf504084z PMid:25514373
Fischer M, Schmidt C, Falke D, Sawers RG. Terminal reduction reactions of nitrate and sulfate assimilation in Streptomyces coelicolor A3(2): identification of genes encoding nitrite and sulfite reductases. Res Microbiol. 2012;163(5):340-8. doi: https://doi.org/10.1016/j.resmic.2012.05.004 PMid:22659143
Mohan P, Lando HA, Panneer S. Assessment of tobacco consumption and control in India. Ind J Clin Med. 2018;9:117991611875928. doi: https://doi.org/10.1177/1179916118759289
National Cancer Institute and Centers for Disease Control and Prevention. 2014. Smokeless tobacco and public health: a global perspective. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention and National Institutes of Health, Bethesda, MD.
Smyth EM, Kulkarni P, Claye E, Stanfill S, Tyx R, Maddox C, et al. Smokeless tobacco products harbor diverse bacterial microbiota that differ across products and brands. Appl Microbiol Biotechnol. 2017;101(13):5391-403. doi: https://doi.org/10.1007/s00253-017-8282-9 PMid:28432442 PMCid:PMC5520664
Tyx RE, Stanfill SB, Keong LM, Rivera AJ, Satten GA, Watson CH. Characterization of bacterial communities in selected smokeless tobacco products using 16S rDNA analysis. PLoS One. 2016;11(1):e0146939. doi: https://doi.org/10.1371/journal.pone.0146939 PMid:26784944 PMCid:PMC4718623
Mehra R, Mohanty V, Balappanavar AY, Kapoor S. Bacterial contamination of packaged smokeless tobacco sold in India. Tob Prev Cessat. 2020;6:11. doi: https://doi.org/10.18332/tpc/115064 PMid:32548348 PMCid:PMC7291906
Han J, Sanad YM, Deck J, Sutherland JB, Li Z, Walters MJ, et al. Bacterial populations associated with smokeless tobacco products. Appl Environ Microbiol. 2016;82(20):6273-83. doi: https://doi.org/10.1128/AEM.01612-16 PMid:27565615 PMCid:PMC5068160
Rubinstein I, Pedersen GW. Bacillus species are present in chewing tobacco sold in the United States and evoke plasma exudation from the oral mucosa. Clin Vaccine Immunol. 2002;9(5):1057-60. doi: https://doi.org/10.1128/CDLI.9.5.1057-1060.2002 PMid:12204959 PMCid:PMC120061
Ayo-Yusuf OA, van Wyk C, van Wyk CW, de Wet I. Smokeless tobacco products on the South African market do not inhibit oral bacterial flora: a pilot study. South Afr J Epidemiol Infect. 2005;20(4):136-9. doi: https://doi.org/10.1080/10158782.2005.11441251
Samuel O, Michael O. Microbial contamination of locally-prepared snuff sold at eke-awka market, anmbra state, Nigeria. Am J Life Sci Res. 2016;4(3):74-7. doi: https://doi.org/10.21859/ajlsr-040301
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Copyright (c) 2022 Sneha D Mallya, Mridula Madiyal, Somya Mullapudi, Varsha Nair, Muralidhar M Kulkarni
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